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a549  (ATCC)


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    Structured Review

    ATCC a549
    A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9091 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epithelial+cell+line+a549/A549/custom%40crm-ccl-185%4010%2E64898%2F2026%2E08%2E02%2E742305
    Average 99 stars, based on 9091 article reviews
    a549 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Modification:

    Article Title: Lectin-Targeted Prodrugs Activated by Pseudomonas aeruginosa for Self-Destructive Antibiotic Release
    Article Snippet: .. The epithelial cell line A549 (ATCC(R) CCL-185) was cultivated in Dulbecco’s modified Eagle’s medium (DMEM) with 10% heat-inactivated fetal calf serum (FCS) and 20 mM l -glutamine at 37 °C and 5% CO 2 . ..

    Article Title: Integrated protein-protein interaction and RNA interference screens reveal novel restriction and dependency factors for a tick-borne flavivirus in its human host
    Article Snippet: .. The simian epithelial cell line Vero (ATCC No. CCL-81), used for propagation and titration of TBEV, and the human epithelial cell line A549 (ATCC No. CCL-185) were cultivated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco BRL Life Technologies, Gaithersburg, MD) supplemented with 5% fetal calf serum (FCS) (Eurobio) and 100 U/ml penicillin and 100 μg/ml streptomycin (both from Gibco BRL Life Technologies). ..

    Titration:

    Article Title: Integrated protein-protein interaction and RNA interference screens reveal novel restriction and dependency factors for a tick-borne flavivirus in its human host
    Article Snippet: .. The simian epithelial cell line Vero (ATCC No. CCL-81), used for propagation and titration of TBEV, and the human epithelial cell line A549 (ATCC No. CCL-185) were cultivated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco BRL Life Technologies, Gaithersburg, MD) supplemented with 5% fetal calf serum (FCS) (Eurobio) and 100 U/ml penicillin and 100 μg/ml streptomycin (both from Gibco BRL Life Technologies). ..

    Infection:

    Article Title: Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of Klebsiella pneumoniae
    Article Snippet: .. To stimulate the bacterial infection of the human cell lines, each dilution of K. pneumoniae culture isolated from patients with bacteremia, pneumonia, and respiratory, ulcer, and other infections were inoculated (50 μL; 2 × 10 6 cells/mL) onto the surface of a monolayer of the cultured 1.8 × 10 5 cells of the epithelial cell line A549 (ATCC CCL-185) derived from lung cancer, while dilutions (50 μL; 2 × 10 6 cells/mL) of K. pneumoniae from patients with UTIs were inoculated on the monolayer of the human epidermoid cell line A431 (CRL-1555, Manassas, VA, USA). ..

    Article Title: Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of Klebsiella pneumoniae .
    Article Snippet: .. To stimulate the bacterial infection of the human cell lines, each dilution of K. pneumoniae culture isolated from patients with bacteremia, pneumonia, and respiratory, ulcer, and other infections were inoculated (50 μL; 2 × 106 cells/mL) onto the surface of a monolayer of the cultured 1.8 × 105 cells of the epithelial cell line A549 (ATCC CCL-185) derived from lung cancer, while dilutions (50 μL; 2 × 106 cells/mL) of K. pneumoniae from patients with UTIs were inoculated on the monolayer of the human epidermoid cell line A431 (CRL-1555, Manassas, VA, USA). ..

    Isolation:

    Article Title: Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of Klebsiella pneumoniae
    Article Snippet: .. To stimulate the bacterial infection of the human cell lines, each dilution of K. pneumoniae culture isolated from patients with bacteremia, pneumonia, and respiratory, ulcer, and other infections were inoculated (50 μL; 2 × 10 6 cells/mL) onto the surface of a monolayer of the cultured 1.8 × 10 5 cells of the epithelial cell line A549 (ATCC CCL-185) derived from lung cancer, while dilutions (50 μL; 2 × 10 6 cells/mL) of K. pneumoniae from patients with UTIs were inoculated on the monolayer of the human epidermoid cell line A431 (CRL-1555, Manassas, VA, USA). ..

    Article Title: Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of Klebsiella pneumoniae .
    Article Snippet: .. To stimulate the bacterial infection of the human cell lines, each dilution of K. pneumoniae culture isolated from patients with bacteremia, pneumonia, and respiratory, ulcer, and other infections were inoculated (50 μL; 2 × 106 cells/mL) onto the surface of a monolayer of the cultured 1.8 × 105 cells of the epithelial cell line A549 (ATCC CCL-185) derived from lung cancer, while dilutions (50 μL; 2 × 106 cells/mL) of K. pneumoniae from patients with UTIs were inoculated on the monolayer of the human epidermoid cell line A431 (CRL-1555, Manassas, VA, USA). ..

    Cell Culture:

    Article Title: Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of Klebsiella pneumoniae
    Article Snippet: .. To stimulate the bacterial infection of the human cell lines, each dilution of K. pneumoniae culture isolated from patients with bacteremia, pneumonia, and respiratory, ulcer, and other infections were inoculated (50 μL; 2 × 10 6 cells/mL) onto the surface of a monolayer of the cultured 1.8 × 10 5 cells of the epithelial cell line A549 (ATCC CCL-185) derived from lung cancer, while dilutions (50 μL; 2 × 10 6 cells/mL) of K. pneumoniae from patients with UTIs were inoculated on the monolayer of the human epidermoid cell line A431 (CRL-1555, Manassas, VA, USA). ..

    Article Title: Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of Klebsiella pneumoniae .
    Article Snippet: .. To stimulate the bacterial infection of the human cell lines, each dilution of K. pneumoniae culture isolated from patients with bacteremia, pneumonia, and respiratory, ulcer, and other infections were inoculated (50 μL; 2 × 106 cells/mL) onto the surface of a monolayer of the cultured 1.8 × 105 cells of the epithelial cell line A549 (ATCC CCL-185) derived from lung cancer, while dilutions (50 μL; 2 × 106 cells/mL) of K. pneumoniae from patients with UTIs were inoculated on the monolayer of the human epidermoid cell line A431 (CRL-1555, Manassas, VA, USA). ..

    Derivative Assay:

    Article Title: Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of Klebsiella pneumoniae
    Article Snippet: .. To stimulate the bacterial infection of the human cell lines, each dilution of K. pneumoniae culture isolated from patients with bacteremia, pneumonia, and respiratory, ulcer, and other infections were inoculated (50 μL; 2 × 10 6 cells/mL) onto the surface of a monolayer of the cultured 1.8 × 10 5 cells of the epithelial cell line A549 (ATCC CCL-185) derived from lung cancer, while dilutions (50 μL; 2 × 10 6 cells/mL) of K. pneumoniae from patients with UTIs were inoculated on the monolayer of the human epidermoid cell line A431 (CRL-1555, Manassas, VA, USA). ..

    Article Title: Extensive Expression of the Virulome Related to Antibiotic Genotyping in Nosocomial Strains of Klebsiella pneumoniae .
    Article Snippet: .. To stimulate the bacterial infection of the human cell lines, each dilution of K. pneumoniae culture isolated from patients with bacteremia, pneumonia, and respiratory, ulcer, and other infections were inoculated (50 μL; 2 × 106 cells/mL) onto the surface of a monolayer of the cultured 1.8 × 105 cells of the epithelial cell line A549 (ATCC CCL-185) derived from lung cancer, while dilutions (50 μL; 2 × 106 cells/mL) of K. pneumoniae from patients with UTIs were inoculated on the monolayer of the human epidermoid cell line A431 (CRL-1555, Manassas, VA, USA). ..

    Incubation:

    Article Title: Human respiratory syncytial virus regulates the expression of interferon-stimulated genes through modulation of fibrillarin.
    Article Snippet: After incubation at 37 °C for 2 h, the inoculum was removed and cells were overlaid with a DMEM medium containing 3% methylcellulose (Sigma- Aldrich, USA). .. Cultures were then incubated for 5 days under the same conditions, and plaques were visualized by staining with napthol-blue black solution to quantify PFUs for subsequent experiments. frontiersin.org Epithelial cell line A549 (ATCC CCL-185TM) was grown in Advanced F12K Medium (Gibco, USA) supplemented with 2 mM glutamine, antibiotics (penicillin 5 × 104 U/mL and streptomycin 50 mg/mL; Gibco), 10% fetal bovine serum (Gibco) and 10 mL/L pyruvate (Gibco, USA) at 37 °C and 5% CO2 atmosphere. .. A total of 4 × 105 A549 cells were transfected with small interfering RNA (siRNA) targeting FBL (Thermo Fisher Scientific #s4820), siRNA targeting p53 (Santa Cruz Biotechnology, #sc29435), or a scrambled siRNA control (Thermo Fisher Scientific #4390843) using LipofectamineTM 2000 reagent, according to the manufacturer’s instructions.

    Staining:

    Article Title: Human respiratory syncytial virus regulates the expression of interferon-stimulated genes through modulation of fibrillarin.
    Article Snippet: After incubation at 37 °C for 2 h, the inoculum was removed and cells were overlaid with a DMEM medium containing 3% methylcellulose (Sigma- Aldrich, USA). .. Cultures were then incubated for 5 days under the same conditions, and plaques were visualized by staining with napthol-blue black solution to quantify PFUs for subsequent experiments. frontiersin.org Epithelial cell line A549 (ATCC CCL-185TM) was grown in Advanced F12K Medium (Gibco, USA) supplemented with 2 mM glutamine, antibiotics (penicillin 5 × 104 U/mL and streptomycin 50 mg/mL; Gibco), 10% fetal bovine serum (Gibco) and 10 mL/L pyruvate (Gibco, USA) at 37 °C and 5% CO2 atmosphere. .. A total of 4 × 105 A549 cells were transfected with small interfering RNA (siRNA) targeting FBL (Thermo Fisher Scientific #s4820), siRNA targeting p53 (Santa Cruz Biotechnology, #sc29435), or a scrambled siRNA control (Thermo Fisher Scientific #4390843) using LipofectamineTM 2000 reagent, according to the manufacturer’s instructions.

    Bacteria:

    Article Title: Enhancing Gentamicin Antibacterial Activity by Co-Encapsulation with Thymoquinone in Liposomal Formulation
    Article Snippet: .. Cell Line and Tested Bacteria We tested the ability of the Lipo-GEN-THQ to prevent the adhesion of E. coli (EC-157) to lung tissue using the pulmonary epithelial cell line A549 (ATCC CCL-185TM, Manassas, VA, USA). .. We seeded 2 × 105 cells/well of A549 on a 24-well plate using Advanced DMEM/F-12 (Gibco™, Grand Island, New York, NY, USA) media supplemented with 10% FBS and 1%L-glutamine (without antibiotics) at 37 ◦C in a humidified 5% CO2 incubator until cells reached confluence.



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    Image Search Results


    Body weight changes of BALB/c mice during acute and repeated-dose toxicity studies of A549-derived extracellular vesicles (EVs) loaded with glucose oxidase (GOX). In the acute toxicity study (A) , mice received a single administration of GOX-loaded EVs at a concentration of 10 8 mL -1 or vehicle (PBS) control, and body weight was monitored over 15 days. This dose was selected as a biologically relevant worst-case exposure to assess acute safety rather than dose-response effects. In the repeated-dose toxicity and immunotoxicity study (B) , mice were treated according to the experimental design described in the Materials and Methods, comprising ten groups (groups 1–10) differing in EV doses, treatment schedule, and respective controls. Body weight was recorded weekly for six weeks. Data are presented as mean ± SD. Statistical analysis revealed no significant differences in body weight between control (group 1) and GOX-loaded EV-treated groups at any time point.

    Journal: Frontiers in Immunology

    Article Title: Systemic toxicity and immunotoxicity studies of glucose oxidase-loaded extracellular vesicles derived from lung cancer cells

    doi: 10.3389/fimmu.2026.1842887

    Figure Lengend Snippet: Body weight changes of BALB/c mice during acute and repeated-dose toxicity studies of A549-derived extracellular vesicles (EVs) loaded with glucose oxidase (GOX). In the acute toxicity study (A) , mice received a single administration of GOX-loaded EVs at a concentration of 10 8 mL -1 or vehicle (PBS) control, and body weight was monitored over 15 days. This dose was selected as a biologically relevant worst-case exposure to assess acute safety rather than dose-response effects. In the repeated-dose toxicity and immunotoxicity study (B) , mice were treated according to the experimental design described in the Materials and Methods, comprising ten groups (groups 1–10) differing in EV doses, treatment schedule, and respective controls. Body weight was recorded weekly for six weeks. Data are presented as mean ± SD. Statistical analysis revealed no significant differences in body weight between control (group 1) and GOX-loaded EV-treated groups at any time point.

    Article Snippet: Human adenocarcinomic alveolar basal epithelial cell line - A549 (ATCC CCL-185) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Derivative Assay, Concentration Assay, Control

    Representative resection specimens of BALB/c mice treated with A549-derived EVs loaded with glucose oxidase based on the acute toxicity protocol (H&E staining, x200, scale bar = 50 μm). PBS-treated control mice (A, C, E, G, I) and GOX-loaded EV-treated mice (B, D, F, H, J) . (A, B) Myocardium: preserved normal myocardial architecture with no evidence of necrosis, degenerative changes, fibrosis, hypertrophy, or inflammatory infiltrates (inflammatory infiltrates grade 0, none). (C, D) Lung: intact alveolar and interstitial architecture with no necrosis, degenerative changes, alveolar damage, emphysema, fibrosis, or inflammatory infiltrates (inflammatory infiltrates grade 0, none). (E, F) Liver: normal hepatic architecture without necrosis, degenerative changes, bile stasis, fibrosis, or inflammatory infiltrates (inflammatory infiltrates grade 0, none). (G, H) Kidney: preserved renal histology with normal glomerular and tubular structure; no necrosis, degenerative changes, fibrosis, or inflammatory infiltrates (inflammatory infiltrates grade 0, none). (I, J) Spleen: normal splenic architecture with no necrosis, degenerative changes, congestion, fibrosis, or white pulp hyperplasia (grade 0, none). Histopathological scoring: Inflammatory infiltrates: 0, none; 0–1, minimal; 1, mild; 2, moderate. White pulp hyperplasia (spleen): 0, none; 0–1, minimal; 1, low; 2, moderate.

    Journal: Frontiers in Immunology

    Article Title: Systemic toxicity and immunotoxicity studies of glucose oxidase-loaded extracellular vesicles derived from lung cancer cells

    doi: 10.3389/fimmu.2026.1842887

    Figure Lengend Snippet: Representative resection specimens of BALB/c mice treated with A549-derived EVs loaded with glucose oxidase based on the acute toxicity protocol (H&E staining, x200, scale bar = 50 μm). PBS-treated control mice (A, C, E, G, I) and GOX-loaded EV-treated mice (B, D, F, H, J) . (A, B) Myocardium: preserved normal myocardial architecture with no evidence of necrosis, degenerative changes, fibrosis, hypertrophy, or inflammatory infiltrates (inflammatory infiltrates grade 0, none). (C, D) Lung: intact alveolar and interstitial architecture with no necrosis, degenerative changes, alveolar damage, emphysema, fibrosis, or inflammatory infiltrates (inflammatory infiltrates grade 0, none). (E, F) Liver: normal hepatic architecture without necrosis, degenerative changes, bile stasis, fibrosis, or inflammatory infiltrates (inflammatory infiltrates grade 0, none). (G, H) Kidney: preserved renal histology with normal glomerular and tubular structure; no necrosis, degenerative changes, fibrosis, or inflammatory infiltrates (inflammatory infiltrates grade 0, none). (I, J) Spleen: normal splenic architecture with no necrosis, degenerative changes, congestion, fibrosis, or white pulp hyperplasia (grade 0, none). Histopathological scoring: Inflammatory infiltrates: 0, none; 0–1, minimal; 1, mild; 2, moderate. White pulp hyperplasia (spleen): 0, none; 0–1, minimal; 1, low; 2, moderate.

    Article Snippet: Human adenocarcinomic alveolar basal epithelial cell line - A549 (ATCC CCL-185) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Derivative Assay, Staining, Control

    Representative resection specimen in BALB/c mice treated with A549-derived EVs loaded with glucose oxidase in repeated dose (28-days) toxicity study (H&E staining, x200, scale bar = 50 μm). PBS-treated control mice: (A) Myocardium: preserved normal architecture; no necrosis, degenerative changes, fibrosis, hypertrophy, or inflammatory infiltrates (inflammatory infiltrates grade 0, none). (C) Lung: intact alveolar and interstitial architecture; no necrosis, degenerative changes, alveolar damage, emphysema, fibrosis, or inflammatory infiltrates (grade 0, none). (E) Liver: normal hepatic architecture; no necrosis, degenerative changes, bile stasis, fibrosis, or inflammatory infiltrates (grade 0, none). (G) Kidney: preserved glomerular and tubular structure; no necrosis, degenerative changes, fibrosis, or inflammatory infiltrates (grade 0, none). (I) Spleen: normal architecture; no necrosis, degenerative changes, congestion, fibrosis, or white pulp hyperplasia (grade 0, none). GOX-loaded EV-treated mice: (B) Myocardium: preserved myocardial fibers with focal chronic inflammatory infiltrates in pericardial adipose tissue (arrows), corresponding to inflammatory infiltrates grade 1 (mild). (D) Lung: chronic peribronchiolar and perivascular inflammatory infiltrates (arrows) with preserved alveolar and interstitial architecture (inflammatory infiltrates grade 1, mild). (F) Liver: normal hepatocyte morphology with mild chronic inflammatory infiltrates in portal spaces (arrows), corresponding to inflammatory infiltrates grade 1 (mild). (H) Kidney: preserved glomeruli and tubules with minimal chronic interstitial inflammatory infiltrates consisting of single, scattered cells (arrows), corresponding to inflammatory infiltrates grade 0–1 (minimal). (J) Spleen: white pulp stimulation with enlarged lymphoid follicles and prominent germinal centers (arrows), corresponding to white pulp hyperplasia grade 2 (moderate). Histopathological scoring: Inflammatory infiltrates: 0, none; 0–1, minimal; 1, mild; 2, moderate. White pulp hyperplasia (spleen): 0, none; 0–1, minimal; 1, low; 2, moderate.

    Journal: Frontiers in Immunology

    Article Title: Systemic toxicity and immunotoxicity studies of glucose oxidase-loaded extracellular vesicles derived from lung cancer cells

    doi: 10.3389/fimmu.2026.1842887

    Figure Lengend Snippet: Representative resection specimen in BALB/c mice treated with A549-derived EVs loaded with glucose oxidase in repeated dose (28-days) toxicity study (H&E staining, x200, scale bar = 50 μm). PBS-treated control mice: (A) Myocardium: preserved normal architecture; no necrosis, degenerative changes, fibrosis, hypertrophy, or inflammatory infiltrates (inflammatory infiltrates grade 0, none). (C) Lung: intact alveolar and interstitial architecture; no necrosis, degenerative changes, alveolar damage, emphysema, fibrosis, or inflammatory infiltrates (grade 0, none). (E) Liver: normal hepatic architecture; no necrosis, degenerative changes, bile stasis, fibrosis, or inflammatory infiltrates (grade 0, none). (G) Kidney: preserved glomerular and tubular structure; no necrosis, degenerative changes, fibrosis, or inflammatory infiltrates (grade 0, none). (I) Spleen: normal architecture; no necrosis, degenerative changes, congestion, fibrosis, or white pulp hyperplasia (grade 0, none). GOX-loaded EV-treated mice: (B) Myocardium: preserved myocardial fibers with focal chronic inflammatory infiltrates in pericardial adipose tissue (arrows), corresponding to inflammatory infiltrates grade 1 (mild). (D) Lung: chronic peribronchiolar and perivascular inflammatory infiltrates (arrows) with preserved alveolar and interstitial architecture (inflammatory infiltrates grade 1, mild). (F) Liver: normal hepatocyte morphology with mild chronic inflammatory infiltrates in portal spaces (arrows), corresponding to inflammatory infiltrates grade 1 (mild). (H) Kidney: preserved glomeruli and tubules with minimal chronic interstitial inflammatory infiltrates consisting of single, scattered cells (arrows), corresponding to inflammatory infiltrates grade 0–1 (minimal). (J) Spleen: white pulp stimulation with enlarged lymphoid follicles and prominent germinal centers (arrows), corresponding to white pulp hyperplasia grade 2 (moderate). Histopathological scoring: Inflammatory infiltrates: 0, none; 0–1, minimal; 1, mild; 2, moderate. White pulp hyperplasia (spleen): 0, none; 0–1, minimal; 1, low; 2, moderate.

    Article Snippet: Human adenocarcinomic alveolar basal epithelial cell line - A549 (ATCC CCL-185) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Derivative Assay, Staining, Control

    Schematic representation of the proposed mechanism of immunomodulation mediated by GOX-loaded EVs based on the TDAR study performed in BALB/c mice. The figure illustrates the impact of GOX-loaded extracellular vesicles (GOX-loaded EVs) on humoral immune responses and the associated cytokine-chemokine milieu required for effective B-cell activation and differentiation. Following in vivo administration, GOX-loaded EVs derived from A549 lung cancer cells interact with the immune system in a constrained and context-dependent manner, without inducing a classical inflammatory response. GOX-loaded EVs elicit a selective chemokine-biased response, characterized by upregulation of Cxcl9 and modest modulation of Cxcl3, Cxcl16, Ccl5, Ccl12, and Ccl17, in the absence of broad cytokine induction. Therefore, B-cell activation and differentiation into antibody-secreting plasma cells remain limited, leading to reduced production of antigen-specific antibodies. At the systemic level, humoral immune analysis reveals an increase in total circulating IgM at later time points, while IgG levels remain unchanged, indicating selective engagement of early humoral response elements without full isotype class switching. GOX-loaded EVs act as immunomodulatory entities, eliciting a selectively confined humoral response and a chemokine-biased immune profile, rather than exhibiting the broad immunostimulatory activity characteristic of classical immune adjuvants.

    Journal: Frontiers in Immunology

    Article Title: Systemic toxicity and immunotoxicity studies of glucose oxidase-loaded extracellular vesicles derived from lung cancer cells

    doi: 10.3389/fimmu.2026.1842887

    Figure Lengend Snippet: Schematic representation of the proposed mechanism of immunomodulation mediated by GOX-loaded EVs based on the TDAR study performed in BALB/c mice. The figure illustrates the impact of GOX-loaded extracellular vesicles (GOX-loaded EVs) on humoral immune responses and the associated cytokine-chemokine milieu required for effective B-cell activation and differentiation. Following in vivo administration, GOX-loaded EVs derived from A549 lung cancer cells interact with the immune system in a constrained and context-dependent manner, without inducing a classical inflammatory response. GOX-loaded EVs elicit a selective chemokine-biased response, characterized by upregulation of Cxcl9 and modest modulation of Cxcl3, Cxcl16, Ccl5, Ccl12, and Ccl17, in the absence of broad cytokine induction. Therefore, B-cell activation and differentiation into antibody-secreting plasma cells remain limited, leading to reduced production of antigen-specific antibodies. At the systemic level, humoral immune analysis reveals an increase in total circulating IgM at later time points, while IgG levels remain unchanged, indicating selective engagement of early humoral response elements without full isotype class switching. GOX-loaded EVs act as immunomodulatory entities, eliciting a selectively confined humoral response and a chemokine-biased immune profile, rather than exhibiting the broad immunostimulatory activity characteristic of classical immune adjuvants.

    Article Snippet: Human adenocarcinomic alveolar basal epithelial cell line - A549 (ATCC CCL-185) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Activation Assay, In Vivo, Derivative Assay, Clinical Proteomics, Activity Assay

    Effect of PsaA-peptide-derived serum on pneumococcal adherence in adhesion assay. Quantification of bacterial adherence to A549 cells. Bacterial adherence to A549 cells following pre-incubation with either anti-S1 or anti-S3 immune sera and normalized to control mouse sera.

    Journal: mSphere

    Article Title: Identification of the Annexin A2-interacting domain of pneumococcal PsaA

    doi: 10.1128/msphere.00232-26

    Figure Lengend Snippet: Effect of PsaA-peptide-derived serum on pneumococcal adherence in adhesion assay. Quantification of bacterial adherence to A549 cells. Bacterial adherence to A549 cells following pre-incubation with either anti-S1 or anti-S3 immune sera and normalized to control mouse sera.

    Article Snippet: Adhesion assays were performed using the human A549 epithelial cell line (ATCC CCL-185).

    Techniques: Derivative Assay, Cell Adhesion Assay, Incubation, Control

    Netilmicin sulfate alone and in combination exerted protective effects on an infected A549 cell model (A) Cytotoxic effects of the drugs on A549 cells. (B) Protective effects of different concentrations of NETS on A549 cells. The early interventions on the abscissa reflect the incubation of A549 cells with the drug for 2 h before B. pseudomallei HNBP001 infection. Late intervention was defined as the introduction of the drug to A549 cells 2 h after infection with B. pseudomallei HNBP001 . The ordinate is the survival rate of the infected A549 cells. NC: the blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Bp+30 μM NETS: The infected A549 cells were treated with 30 μM NETS; the other conditions were the same, but different concentrations of NETS were used. (C) Protective effects of several drugs on infected A549 cells; the ordinate is the survival rate of infected A549 cells. NC, blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Other: The infected A549 cells were treated with drugs (including SXT, GM, NETS, CAZ, and NETS+CAZ). (D) Number of intracellular bacteria in infected A549 cells after drug treatment; the ordinate represents the number of bacteria in infected A549 cells. NC is the blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Drug group: 2 × MIC drug was used to treat infected A549 cells. All drugs, including SXT, GM, NETS, CAZ, and NETS+CAZ. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001; ns, no significant difference. Data are represented as mean ± SEM.).

    Journal: iScience

    Article Title: Drug screening to identify compounds to eliminate Burkholderia pseudomallei through Hcp protein

    doi: 10.1016/j.isci.2026.115367

    Figure Lengend Snippet: Netilmicin sulfate alone and in combination exerted protective effects on an infected A549 cell model (A) Cytotoxic effects of the drugs on A549 cells. (B) Protective effects of different concentrations of NETS on A549 cells. The early interventions on the abscissa reflect the incubation of A549 cells with the drug for 2 h before B. pseudomallei HNBP001 infection. Late intervention was defined as the introduction of the drug to A549 cells 2 h after infection with B. pseudomallei HNBP001 . The ordinate is the survival rate of the infected A549 cells. NC: the blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Bp+30 μM NETS: The infected A549 cells were treated with 30 μM NETS; the other conditions were the same, but different concentrations of NETS were used. (C) Protective effects of several drugs on infected A549 cells; the ordinate is the survival rate of infected A549 cells. NC, blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Other: The infected A549 cells were treated with drugs (including SXT, GM, NETS, CAZ, and NETS+CAZ). (D) Number of intracellular bacteria in infected A549 cells after drug treatment; the ordinate represents the number of bacteria in infected A549 cells. NC is the blank negative control group of fresh 10% DMEM. Bp: infected A549 cell group. Drug group: 2 × MIC drug was used to treat infected A549 cells. All drugs, including SXT, GM, NETS, CAZ, and NETS+CAZ. (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001; ns, no significant difference. Data are represented as mean ± SEM.).

    Article Snippet: The A549 human lung carcinoma epithelial cell line was purchased from Procell (Wuhan, China; Cat# CL-0016).

    Techniques: Infection, Incubation, Negative Control, Bacteria